Laboratoire de Physique et Chimie des Nano-objets

Institut National des Sciences Appliquées
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Home page > LPCNO > Seminars > 2015 > Lab on Chips for bio-molecular and cellular analysis : from droplet immunoassays to rare cell sorting.

Lab on Chips for bio-molecular and cellular analysis : from droplet immunoassays to rare cell sorting.

Date : 19/11/2015 à 14:00

Titre : Lab on Chips for bio-molecular and cellular analysis : from droplet immunoassays to rare cell sorting.

Intervenant : Laurent Malaquin

Provenance : LAAS, Toulouse

Salle : Salle de Séminaire - LPCNO

Résumé: Microfluidics and nanofluidics have shown considerable promise for improving the fields of biomedical science and clinical diagnostics. After undergoing its own development and establishment as a technology over past years, microfluidics is now being increasingly applied to areas such as biomarker analysis where it has the potential to transform the field. Microfluidics offers the possibility to select, manipulate, stimulate, individual cells or even molecules, in a highly parallelized and automated manner. It thus provides all the basic functions that are required to perform the complex analytical workflows leading to biomarker detection and quantitation. In this talk, I will present examples of integrated devices developed for the capture, pre-concentration and analysis of biomarkers. I will show how magnetic forces and self-assembly processes can be advantageously tuned and combined with hydrodynamic concepts to create functional assemblies of particles in microfluidic devices. I will describe three examples of bio-analytical applications that we recently developed for i) the integration of functionalized 3D structures for protein detection and capture of rare cancerous cells ii) the generation of a miniaturized fluidized bed for bio molecular and bacteria pre-concentration iii) the combination of droplet microfluidics and magnetic tweezers for multiplexed heterogeneous immuno-assays and transcriptomic analysis through RT PCR.